The UCSD Department of Pharmacology is one of two basic science units in the School of Medicine where world-class researchers investigate the effects of chemical interventions on biological systems. The faculty are internationally renowned for their strength in elucidating basic cellular and molecular mechanisms of signal transduction that lead to innovative drug target identification and ultimately to cutting edge drug discovery. The faculty also play a major role in medical and graduate student training, providing educational and programmatic offerings that span several disciplines, and provide diversity to meet the interests of a broad spectrum of students and scholars. More than one hundred trainees at the graduate student and postdoctoral level, as well as a staff of more than 50 research technicians and department administrators interact closely with the faculty. The diverse mix of ages, backgrounds, and talents creates arobust work environment with challenging career opportunities and a commitment to continued growth potential. We constantly seek to recruit highly motivated, technologically-advanced and interested individuals to become a part of our dynamic exploratory research and educational environment. For more information, please visit our website at http://pharmacology.ucsd.edu.

Under the general supervision of the laboratory Principal Investigator, the incumbent will perform a variety of experimental procedures for ongoing laboratory research projects. Research will utilize microbiology, cloning, and mass spectrometry procedures. The incumbent will complete experimental research by operating various laboratory equipment and maintain all data analysis using the associated software. Incumbent will also assist the PI with laboratory management to include; EH&S compliance, maintenance of key laboratory equipment/machines, and ordering supplies.

• Theoretical knowledge of chemistry, biochemistry, cloning, and computer science or demonstrated equivalent of combined education and experience.

• Proven ability to independently participate in the execution of research projects or portions of such projects, through the independent planning, organizing and performing of experiments.

• Knowledge of standard laboratory procedures in reference to working with various chemicals including acid, and bacteria, especially pathogenic strains, including liquid-liquid extractions, culturing, and genetic manipulation. Ability to prepare and maintain media buffers and mathematical skills to perform molar calculations.

• Demonstrated ability to properly use and maintain laboratory equipment including: centrifuges, pH meters, spectrophotometers, balances, computers, etc.

• Knowledge of qPCR thermocycler instrumentation, operation, and maintenance for Eppendorf RealPlex 4.

• Knowledge of mass spectrometry instrumentation, operation, and maintenance for Dionex Ultimate 3000 HPLC.

• Proficiency in the application of softwares including Chromeleon, Xcalibur, Proteome Discoverer, and Cytoscape.

• Ability to keep detailed and understandable records of experimental procedures, results, calculations, and evaluation of research results.

• Ability to utilize multiple types of computer programs for performance of scientific equipment, recording of data, data analyses, statistical evaluation, graphical presentation of results, written descriptions of research, and illustrations of research topics.

• Experience working in a fast-paced research laboratory, ability to simultaneously make progress on several related projects, ability to troubleshoot and modify protocols to meet deadlines.

• Experience in training, providing direction and supervision to other lab personnel and students.

• Ability to coordinate protein needs of the laboratory.

• Demonstrated ability to independently carry out statistical analysis and draw independent scientific conclusions.

• Employment is subject to a criminal background check.

• Must be willing to work a flexible schedule to accommodate protein purification. Work in cold room (4 degrees C).