The Department of Anesthesiology employs over 200 academic and staff personnel and has an annual operating budget of $40 million in annualized resources between Practice Plan Revenues, VAMC funding, Hospital and Medical Group Funding, UC Foundation, Research Sources and School of Medicine Funds. There are over 80 clinical faculty, 12 Ph.D, faculty and post docs, 41 residents, 16 clinical fellows, 27 Certified Nurse Anesthetists, 1 Physician Assistant and four bargaining units represented. The Department provides Clinical Anesthesia, Critical Care and Pain Services at Hillcrest, Thornton, Shiley, Children's and the VAMC. The Department operates a Pain Clinic at Perlman. The Department's research programs include bench and clinical research programs located at the VAMC, MTF, CTF, BSB, Hillcrest, Thornton and Sanford Consortium for Regenerative Medicine.

Under supervision of the Principal Investigator, incumbent will perform a variety of cellular and molecular techniques. Responsibilities include preparation of RNA for next generation sequencing, preparing and maintaining primary cell cultures, immunohistochemistry, FACS sorting and confocal imaging. The position requires detail-oriented observations and accurate record keeping. Responsible for providing the Principle Investigator with a written assessment of experimental outcomes. Participates in the planning of new and existing experiments, by studying available literature, then analyzing, troubleshooting and altering conditions under which determinations are made. Responsible for general laboratory maintenance including organization of lab materials, maintaining laboratory databases, placing supply orders, drafting and maintaining animal protocols and assisting with maintaining laboratory equipment. Willingness to teach and collaborate with PhD and Master level graduate students and visiting scholars in technical studies.

• Theoretical knowledge of a life science as typically acquired by a Bachelor's degree in the field of molecular biology, biochemistry, biotechnology, bioengineering or genetics or an equivalent combination of education and experience.

• Experience preparing and maintaining tissue derivation, ESC derivation, micro-dissection, tissue cultures. Knowledge of sterile techniques and culture media/reagents. Demonstrated ability to perform routine RNA isolation and analysis. Experience with basic protein chemistry techniques.

• Ability to maintain supplies and consumables for medium size laboratory and its core facilities including meticulous record keeping of ordered and received materials.

• Experience in maintaining general laboratory equipment (e.g. centrifuges, water baths, etc. and specialized equipment (i.e., OROBOROS, Seahorse, Immunofluorescence, Confocal Microscopy)

• Knowledge of EH&S procedures for laboratory safety.

• Intermediate to advance computer skills using MS Word, Excel, PowerPoint, Graphpad Prism, Imaris, Image email system and a variety of web based programs (Genbank).

• Ability to efficiently manage time and organizational skills. Ability to establish priorities, meet multiple and frequently changing deadlines with flexibility. Detail oriented.

• Experience and willingness to work with animals.

• Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment.

• Ability to assist lab personnel, maintain and order supplies, coordinate research projects, train personnel on research techniques and use laboratory equipment.

• Knowledge of surgical techniques to aid in the study of nerve regeneration and neuropathic pain in vitro and in vivo.

• Previous experience working in a mitochondrial laboratory, with stem cell culture & iPS cells.

• Knowledge of assessing behaviors in animals.

• Knowledge of assessing mitochondrial function in tissue and cell samples and ability to analyze this data using Prism software.

• Ability to work with and manage large data sets to communicate with study participants and staff.

• Employment is subject to a criminal background check.

• Must be willing to work with animals.

• Must be willing to work with bio-hazardous materials.

• Must be willing to work occasional weekends or extended hours to meet project deadlines and experiments.

• Must be willing to work with Visiting scholars with minimal English proficiency.