A postdoctoral position is available to work in the laboratory of the Director at the Center for Blood Oxygen Transport and Hemostasis (CBO2TH) at the University of Maryland, Baltimore (UMB). Dr. Allan Doctor is seeking a highly motivated, dedicated, and enthusiastic young scientist, with primary interest in oxygen transport and vasoregulatory controls, with particular focus on red blood cell signaling and physiology. An opening exists to study acquisition of abnormal red blood cell phenotype resulting from RBC storage, and the impact of this abnormal phenotype upon oxygen transport and vasoregulation.
During storage, RBCs undergo significant biochemical/morphologic changes, resulting in a phenotype referred to as the “storage lesion”. The Doctor Lab hypothesize that this phenotype may arise from disruption of oxygen-based regulation of RBC energy metabolism, with resultant depowering of intrinsic antioxidant systems and functionally significant modification to cytosolic, skeletal and membrane proteins. Such protein damage likely impairs donor RBC oxygen transport efficacy, vasoregulatory control, and reduces the lifespan of the stored RBCs following transfusion.
This project will provide access to both human specimens and state-of-the-art animal model systems, with focus upon high resolution mapping/analysis of RBC energy metabolism and oxygen transport at the organism, organ system, microcirculatory and cellular level. Additional murine model systems are hoped to be developed, including a nude mouse model to allow in vivo efficacy testing of human RBCs, and a GFP mouse model to allow label free assessment of RBC lifespan and clearance. Ultimetely, it is hoped that this work will inform new strategies to mitigate the storage lesion and extend RBC shelf life.
The position will be integrated into comprehensive scientific training environment at the newly formed UMB Research Center – with immersive interaction involving multiple research teams focused upon Oxygen Transport and Hemostasis.
- Perform/optimize advanced laboratory research analysis of RBCs (metbolomic profiling, RBC membrane proteomics, flow cytometry, oxygen dissociation curves, deformability, adhesion, aggregation, bioassays of vascular reactivity, ELISAs) and measurement of tissue oxygenation (phosphorescence quenching, animal imaging - bioluminescence).
- Perform/optimize advanced analytical chemistry assays to quantify RBC-based control of vasoactive effectors in plasma (Nitric Oxide metabolites, Adenosine/ATP metabolites, and etc.).
- Perform advanced laboratory animal research activities, such as exchange blood transfusions, serial blood draws, animal imaging.
- Compile and analyze research data using various relevant computer software applications (Graphpad Prism, Excel, Gen5, Clarity, Unicorn, LabChart)
- Perform in-depth literature research, prepare high impact peer-reviewed manuscripts, and contribute to grant proposals.
- Present findings at scientific meetings
- Perform administrative duties such as training, mentoring and guiding students and/or other laboratory staff; assist in developing policies, procedures, protocols and/or methods for laboratory experimentation
- Perform other duties as assigned
- PhD. In Biochemistry, Cell Biology, Physiology or a related field
- Be able to work independently in a fast-paced dynamic research environment
- Have very strong oral and written communication skills
- Work well in a team environment and in a collaborative setting
Experience in related laboratory work.
To apply, candidates should send (1) a cover letter (describing their experience, goals, and reasons for interest in this position), (2) curriculum vitae, and (3) the contact information of three references as a single combined PDF to Dr. Doctor.
TO APPLY: Please send an email Dr. Doctor at ADoctor@som.umaryland.edu